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Fig. 6. Pro-osteoclastic capacities of supramolecular peptide nanofiber hydrogels. (A) Live/dead staining for cell viability and Transwell assay for cell migration of RAW264.7 cells, scale bar: 200 μm (up) and 100 μm (down). (B) Tartrate-resistant acid phosphatase (TRAP) staining and F-actin ring immunofluorescence staining for osteoclastogenesis of RAW264.7 cells, scale bar: 100 μm. (C) Migration quantitative analysis, (D) TRAP-positive osteoclast quantitative analysis, (E) F-actin ring quantitative analysis and osteoclastogenic-related gene expression (F) <t>NFATc1,</t> (G) CTSK, (H) RANK, (I) TRAP and (J) DC-STAMP of RAW264.7 cells. Immuno fluorescence staining of (K) NFATc1 and (L) CTSK, scale bar: 100 μm *P < 0.05, **P < 0.01, and ****P < 0.0001 indicate significant difference compared to 0% P1R16; #P < 0.05, ##P < 0.01, and ####P < 0.0001 indicate significant difference compared to 50% P1R16.
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Fig. 6. Pro-osteoclastic capacities of supramolecular peptide nanofiber hydrogels. (A) Live/dead staining for cell viability and Transwell assay for cell migration of RAW264.7 cells, scale bar: 200 μm (up) and 100 μm (down). (B) Tartrate-resistant acid phosphatase (TRAP) staining and F-actin ring immunofluorescence staining for osteoclastogenesis of RAW264.7 cells, scale bar: 100 μm. (C) Migration quantitative analysis, (D) TRAP-positive osteoclast quantitative analysis, (E) F-actin ring quantitative analysis and osteoclastogenic-related gene expression (F) <t>NFATc1,</t> (G) CTSK, (H) RANK, (I) TRAP and (J) DC-STAMP of RAW264.7 cells. Immuno fluorescence staining of (K) NFATc1 and (L) CTSK, scale bar: 100 μm *P < 0.05, **P < 0.01, and ****P < 0.0001 indicate significant difference compared to 0% P1R16; #P < 0.05, ##P < 0.01, and ####P < 0.0001 indicate significant difference compared to 50% P1R16.
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Fig. 6. Pro-osteoclastic capacities of supramolecular peptide nanofiber hydrogels. (A) Live/dead staining for cell viability and Transwell assay for cell migration of RAW264.7 cells, scale bar: 200 μm (up) and 100 μm (down). (B) Tartrate-resistant acid phosphatase (TRAP) staining and F-actin ring immunofluorescence staining for osteoclastogenesis of RAW264.7 cells, scale bar: 100 μm. (C) Migration quantitative analysis, (D) TRAP-positive osteoclast quantitative analysis, (E) F-actin ring quantitative analysis and osteoclastogenic-related gene expression (F) NFATc1, (G) CTSK, (H) RANK, (I) TRAP and (J) DC-STAMP of RAW264.7 cells. Immuno fluorescence staining of (K) NFATc1 and (L) CTSK, scale bar: 100 μm *P < 0.05, **P < 0.01, and ****P < 0.0001 indicate significant difference compared to 0% P1R16; #P < 0.05, ##P < 0.01, and ####P < 0.0001 indicate significant difference compared to 50% P1R16.

Journal: Bioactive materials

Article Title: A parathyroid hormone related supramolecular peptide for multi-functionalized osteoregeneration.

doi: 10.1016/j.bioactmat.2023.12.014

Figure Lengend Snippet: Fig. 6. Pro-osteoclastic capacities of supramolecular peptide nanofiber hydrogels. (A) Live/dead staining for cell viability and Transwell assay for cell migration of RAW264.7 cells, scale bar: 200 μm (up) and 100 μm (down). (B) Tartrate-resistant acid phosphatase (TRAP) staining and F-actin ring immunofluorescence staining for osteoclastogenesis of RAW264.7 cells, scale bar: 100 μm. (C) Migration quantitative analysis, (D) TRAP-positive osteoclast quantitative analysis, (E) F-actin ring quantitative analysis and osteoclastogenic-related gene expression (F) NFATc1, (G) CTSK, (H) RANK, (I) TRAP and (J) DC-STAMP of RAW264.7 cells. Immuno fluorescence staining of (K) NFATc1 and (L) CTSK, scale bar: 100 μm *P < 0.05, **P < 0.01, and ****P < 0.0001 indicate significant difference compared to 0% P1R16; #P < 0.05, ##P < 0.01, and ####P < 0.0001 indicate significant difference compared to 50% P1R16.

Article Snippet: Primary antibodies against NFATc1 (Proteintech, USA) and CTSK (Proteintech, USA) at a dilution of 1:100 were added to incubate samples at 4 ◦C overnight.

Techniques: Staining, Transwell Assay, Migration, Immunofluorescence, Gene Expression, Fluorescence